Methods: Search Results

  1. Basal Cytotoxicity (17 Results)

    Protocol no 36

    The inhibition of CHO cell proliferation provides an overall assessment of the toxicity of the test substance.

    Contact Person: Dr. Cascorbi Ingolf

    Status: Participation in evaluation studies

  2. Protocol no 35

    The effect of chemicals on the activity of the plasma membrane-bound Na+/K+ -ATPase isolated from Chinese Hamster Ovary (CHO) cells is used as a measure of their toxicity.

    Contact Person: Dr. Cascorbi Ingolf

    Status: Participation in evaluation studies

  3. Protocol no 24

    Changes in the balance of cytoskeletal proteins after exposure to test compounds can be detected by indirect immunofluorescence microscopy and quantitative biochemical methods.

  4. Protocol no 14

    The ability of cultured cells to synthesise protein is used to assess the effect of a test compound on cellular anabolic competence.

    Contact Person: Dr. Marinovich Marina

  5. Protocol no 6

    This method measures the leakage of DNA and lactate dehydrogenase (LDH, EC. 1.1.1 27) from lymphocytes into the surrounding medium as an indicator of cytotoxicity. This method also includes an assay of intracellular (mitochondrial) diaphorase as a measure of cellular activity (MTT assay).

    Contact Person: Prof. Dr. Clausen Jorgen

    Status: Participation in evaluation studies

  6. Protocol no 38

    This simple cell culture-based cytotoxicity test (in which cell viability is determined by uptake of the dyes ethidium bromide and fluorescein acetate) has been developed as a general test for acute toxicity.

    Further Applications: Eye Irritation

    Contact Person: Dr. Kemp R.B.

  7. Protocol no 25

    Tumour cell lines cultured as aggregates can be utilised for in vitro radiosensitivity and/or chemosensitivity tests. Chemical effects are monitored by studying the changes in spheroid diameter measured by laser diffraction.

    Further Applications: Drug Discovery and Activity Testing

    Contact Person: Dr. Dyson J.E.

  8. Protocol no 17

    This protocol provides a generic description of a simple assay, which can be used to determine the viability/number of cells in culture. The qunatitative measurement is made through a formation of a coloured product (in a mitochondria-dependent reaction) to which the cell membrane is impermeable.

    Contact Person: Dr. Supino Rosanna

    Status: Participation in evaluation studies

  9. Protocol no 52

    AVEC-DIC microscopy in combination with mitochondria-specific fluorescence allows a quantitative analysis of cell organelle dynamics and fine structure in cell cultures exposed to test compounds.

    Contact Person: Lindl Toni

  10. Protocol no 15

    The cytotoxic effect of chemicals upon cells in culture is measured by the change in total cell protein arising from the inhibition of cell proliferation (Kenacid Blue R dye-binding method).

    Contact Person: Dr. Clothier Richard

  11. Protocol no 3

    The cytotoxic effect of chemicals upon cells in culture is measured by cell viability (neutral red uptake) method.

    Further Applications: Eye Irritation

    Contact Person: Dr. Clothier Richard

  12. Protocol no 9

    Membrane permeability of perfused cell cultures, as determined by the efflux of [3H]-2-deoxy-D-glucose-6-phosphate, is used as an indicator of the cytotoxic effect of chemicals.

    Contact Person: Dr. Walum Erik

  13. Protocol no 73

    The activating system (human liver microsomes) is separated by a semi-permeable membrane from the target cells (human mononuclear leucocytes or red cells) in order to identify cytotoxic metabolites that are capable of diffusing away from the site of production.

    Further Applications: Hepatotoxicity / Metabolism-mediated Toxicity

    Contact Person: Dr. Tingle M.D.

  14. Protocol no 58

    The absorption of UV at 260nm in a fixed volume of solubilised cells is proportional to the cell number, and therefore can be used as a simple means of obtaining a cell count. Cell counts obtained in this way can be combined with measurements of the inhibition of DNA synthesis ([3H]-thymidine incorporation) by test compounds, to produce an index of cytotoxicity.

    Contact Person: Dr. Chang Ming J.W.

  15. Protocol no 39

    The cytotoxic effect of test chemicals in V79 cell culture can be determined by assessing damage to the plasma membrane as determined by a nucleic acid leakage assay.

    Contact Person: Prof. Dr. Bianchi Vera

  16. Protocol no 33

    The cytotoxic effect of chemicals upon yeast (Saccharomyces cerevisiae) cells in culture is determined by inhibition of cell proliferation, as measured by cell density.

    Contact Person: Dr. Cascorbi Ingolf

    Status: Participation in evaluation studies

  17. Protocol no 34

    The effect of chemicals on the activity of the plasma membrane-bound H+-ATPase, isolated from yeast (Saccharomyces cerevisiae) cells, is used as a measure of their toxicity.

    Contact Person: Dr. Cascorbi Ingolf

    Status: Participation in evaluation studies

  18. Biocompatibility & Safety of Medical Devices (3 Results)

    Protocol no 50

    Two cytotoxicity tests are used in parallel to investigate the toxicity of implant materials used in medicine and dentistry.

    Contact Person: Dr. Miroslav Cervinka

  19. Protocol no 4

    This method enables the in vitro cytotoxicity testing of dental restorative materials which may then be related to dental toxicity likely to occur in vivo.

    Status: National standard - British Standard

  20. Protocol no 104

    The toxicity of biomaterials is assessed over a seven-day exposure to cells in a semi-solid medium.

    Contact Person: Dr. van Luyn M.J.A.

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